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SJTU Research Team Made Progress on Studying Liquid Phase Biochip

December 02, 2019      Author: Wu Weijie

Recently, Researcher Li Wanwan’s team from SJTU State Key Laboratory of Metal Matrix Composites has made great progress on the study of liquid phase biochip and will publish the research paper Precisely Encoded Barcodes Using Tetrapod CdSe/CdS Quantum Dots with a Large Stokes Shift for Multiplexed Detection on Advanced Functional Materials (2018 IF: 15.621). The paper will be highlighted on the back cover of the journal. Doctoral student Wu Weijie from Researcher Li’s team is the first author, and Researcher Li is the corresponding author.

In recent years, Li’s team has concentrated on the key and tricky issue in liquid phase biochip examination and made many advancements. The paper to be published demonstrates an effective and simple way to eliminate energy transfer, which is important for the preparation of fluorescent encoded microspheres and provides other areas with a new way of eliminating polychromatic luminescent material energy transfer.

 

This paper was also supported by Prof. Shen Lisong’s team from the Clinical Laboratory of SJTU Xinhua Hospital and Prof. Li Li’s team from the Clinical Laboratory of Shanghai General Hospital, and funded by National Key Research and Development Project, National Natural Science Foundation of China and Science and Technology Commission Shanghai Municipality

Abstract

 

A serious obstacle to the construction of high‐capacity optical barcodes in suspension array technology is energy transfer, which can prompt unpredictable barcode signals, limited barcode numbers, and the need for an unfeasible number of experimental iterations. This work reports an effective and simple way to eliminate energy transfer in multicolor quantum dots (QDs)‐encoded microbeads by incorporating tetrapod CdSe/CdS QDs with a large Stokes shift (about 180 nm). Exploiting this unique feature enables the facile realization of a theoretical 7 × 7‐1 barcoding matrix combining two colors and seven intensity levels. As such, microbeads containing tetrapod CdSe/CdS QDs are demonstrated to possess a powerful encoding capacity which allows for precise barcode design. The ability of the Shirasu porous glass membrane emulsification method to easily control microbead size facilitates the establishment of a 3D barcode library of 144 distinguishable barcodes, indicating the enormous potential to enable large‐scale multiplexed detection. Moreover, when applied for the multiplexed detection of five common allergens, these barcodes exhibit superior detection performance (limit of detection: 0.01–0.02 IU mL1) for both spiked and patient serum samples. Therefore, this new coding strategy helps to expand barcoding capacity while simultaneously reducing the technical and economic barriers to the optical encoding of microbeads for high‐throughput multiplexed detection.

 

Link of paper: https://onlinelibrary.wiley.com/doi/10.1002/adfm.201906707